Method and system for utilizing somatic cell nuclear...

A - Human Necessities – 01 – K

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A01K 67/027 (2006.01) C12N 15/00 (2006.01)

Patent

CA 2509465

The present invention provides method to improve the efficiency of both serial nuclear transfer and the consequent production of transgenic cells and/or animals. This new method, through the use of at least a second round of nuclear transfer, will enable donor cells to correct their reprogramming more efficiently generating larger numbers of useful transgenic embryos. Because the reprogramming of the embryos generated is enhanced the current invention will allow for the production of healthier transgenic animals.

La présente invention fournit un procédé améliorant l'efficacité à la fois du transfert nucléaire en série et de la production conséquente des cellules transgéniques et/ou d'animaux. Ce procédé, par l'utilisation au moins d'un deuxième cycle de transfert nucléaire, permet aux cellules donatrices de corriger leur reprogrammation de manière plus efficace produisant des nombres plus importants d'embryons transgéniques utiles. Puisque la reprogrammation des embryons produits est améliorée l'invention permet la production d'animaux transgéniques plus sains.

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