Method and test kit for analyzing dns repair

Details Method and test kit for analyzing dns repair Method and test kit for analyzing dns repair

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2348937

The invention relates to a method and test kit for analyzing the repair of DNS modifications and the mispairing of bases such as apurinic sites and apyrimidinic sites by DNS repair enzymes. According to said method, DNS molecules that are co-valently coupled to a solid phase matrix by means of reaction with a reactive squaric acid are brought into contact with a composition containing a DNS repair enzyme. The test kit contains the components that are required for said analysis.

L'invention concerne un procédé et une trousse de test pour l'analyse de la réparation de modifications d'ADN et d'appariements défectueux de bases, ainsi que des sites apurine et apyrimidine, au moyen d'enzymes de réparation d'ADN. Selon ledit procédé, des molécules d'ADN qui sont, par réaction avec un dérivé réactif de C¿4?H¿2?O¿4?, couplés de façon covalente avec une matrice en phase solide sont mises en contact avec une composition contenant des enzymes de réparation d'ADN. La trousse de test comprend les composants nécessaires à la réalisation de cette analyse.

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