Method and test kit for demonstrating genetic identity

Details Method and test kit for demonstrating genetic identity Method and test kit for demonstrating genetic identity

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2472153

Method and kit for demonstrating genetic identity, genetic diversity, genomic variations or polymorphisms, especially allelic variations, and also biodiversity within a defined population pool, with co-dominant scoring. The method and the test kit apply mobile elements (MEs), such as transposons or retrotransposons, and are based on the use of one or more sets of optionally paired or parallel oligonucleotides, which are attached to a solid support. Each oligonucleotide sequence represents an insertion site junction of a mobile element. The invention is also related to the use of the method and kit for phylogenetic studies, parenthood determinations, genotyping, haplotyping, pedigree analysis, forensic science, human medical diagnostics and in plant and animal breeding by demonstrating genetic identity, genetic diversity, genomic variation or polymorphism, and particularly providing co-dominant scoring.

L'invention concerne un procédé et un kit permettant de déterminer, par cotation codominante, l'identité génétique, la diversité génétique, les variations génomiques ou les polymorphismes, particulièrement les variations alléliques, ainsi que la biodiversité dans un pool de population défini. Ces procédé et kit d'essai font intervenir des éléments mobiles (ME), tels que les transposons ou rétrotransposons, et reposent sur l'utilisation d'une ou de plusieurs séries d'oligonucléotides éventuellement appariés ou parallèles, fixés sur un support solide. Chaque séquence d'oligonucléotide comporte une jonction de site d'insertion d'un élément mobile. Par ailleurs, l'invention concerne l'utilisation de ces procédé et kit dans les études phylogéniques, la détermination des parents, le génotypage, l'haplotypage, l'étude du pedigree, la médecine légale, le diagnostic médical humain ainsi que dans la sélection des plantes et des animaux au moyen de la détermination de l'identité génétique, de la diversité génétique, de la variation génétique ou du polymorphisme, particulièrement par cotation codominante.

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