Method and vector organism for transported cloned...

Details Method and vector organism for transported cloned... Method and vector organism for transported cloned...

C - Chemistry, Metallurgy

12

P

195/1.2, 195/1.2

C12P 21/00 (2006.01) C12N 9/86 (2006.01) C12N 15/00 (2006.01) C12N 15/62 (2006.01) C12N 15/75 (2006.01)

Patent

CA 1182408

ABSTRACT A method and a cloning vector are described for transported cloned heterologous gene products in Bacillus subtilis. The cloning vector is capable of being replicated in B. subtilis and includes the heterologous gene located and oriented such as to be under the control of an operator, promoter, and ribosomal binding site sequence. The gene codes for a protein which is under the control of a transport mechanism by which the protein is secreted by the B. subtilis. The gene product is recovered from the growth medium for the B. subtilis. The cloning vector is also capable of similar use in other bacteria such as E. coli.

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