G - Physics – 01 – N
Patent
G - Physics
01
N
167/139, 167/43
G01N 33/53 (2006.01) G01N 21/64 (2006.01) G01N 33/533 (2006.01)
Patent
CA 1082106
ABSTRACT OF THE DISCLOSURE A method is disclosed whereby the molecules of a chemical substance to be measured, referred to as target molecules, are combined with fluorescent molecules having a relatively long fluorescent decay lifetime. These tagged target molecules are excited with an intense pulse of ultra- violet or visible radiation, and a detection system is gated on only after background fluorescence has substantially de- cayed, but while the fluorescent tag attached to the target molecules is still actively decaying. The amount of fluorescent tag emission which is measured is indicative of the target molecule content of the sample An apparatus for improved fluorescent spectrofluorometry includes a pulsed excitation source and a gating means connected to a detection system for gating the detection system on after tagged target molecules have been excited and competing back ground fluorescence has substantially decayed, but before tagged target molecules have substantially decayed. A new family of fluorescent antibodies (FAB) and a method for their synthesis is disclosed for use in an apparatus and method wherein the effects of background flu- orescence are virtually eliminated from fluorescence measure- ments. The novel fluorescent antibodies consist of anit- bodies conjugated with rare-earth chelates which have unique fluorescent properties.
255924
Hodgson Keith O.
Wieder Irwin
Analytical Radiation Corporation
Smart & Biggar
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