Method comprising the indirect electrochemical regeneration...

C - Chemistry – Metallurgy – 12 – P

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C12P 1/00 (2006.01) C12M 1/00 (2006.01) C12P 7/22 (2006.01) C25B 3/04 (2006.01) C25B 9/00 (2006.01)

Patent

CA 2409339

The invention relates to a method comprising the indirect electrochemical regeneration of NAD(P)H in enzymatic substrate reactions, that are, for example, catalyzed by monooxygenases. The invention especially relates to a method for the electroenzymatic production of 2,3-dihydroxyphenyl derivatives that is catalyzed by the enzyme 2-hydroxybiphenyl-3-monooxygenase, while at the same time the NAD+ produced by reductive oxygen separation is electrochemically reduced.

La présente invention concerne un procédé impliquant la régénération électrochimique indirecte de NAD(P)H lors de transformations de substrats enzymatiques qui sont, par exemple, catalysés par des mono-oxygénases. L'objectif de l'invention est en particulier un procédé de production électro-enzymatique de dérivés 2,3-dihydroxyphényle, pour lesquels la catalyse est réalisée au moyen de l'enzyme 2-hydrobiphényl-3-mono-oxygénase, le NAD?+¿ étant, simultanément, réduit électrochimiquement par division réductrice de l'oxygène.

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