Method, device and kit for measurement of tissue plasminogen...

C - Chemistry – Metallurgy – 12 – P

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150/11, 167/45,

C12P 21/08 (2006.01) C07K 16/40 (2006.01) C12Q 1/37 (2006.01) C12Q 1/56 (2006.01) G01N 33/573 (2006.01) G01N 33/577 (2006.01)

Patent

CA 1331158

- 50 - ABSTRACT OF THE DISCLOSURE A true activity of tissue plasminogen activator is determined by reacting a solid phase having bound thereto anti tissue plasminogen activator antibody IgG fragment having affinity to a site other than the active site of tissue plasminogen activator with tissue plasminogen activator in a sample and then measuring an activity of the reaction product, preferably in terms of an amount of a substance released from a chromogenic peptide substrate. This measurement enables to use of whole blood or diluted blood as the sample and achieves high sensitivity and high accuracy in a short period of time.

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