Method for a continuous rapid thermal cycle system

C - Chemistry – Metallurgy – 12 – P

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C12P 19/34 (2006.01) C12M 1/38 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2713101

An efficient, high speed production scale synthesis method for high molecular weight organic substances such as DNA by methods which require multiple temperature environments. In a Polymerase Chain Reaction operation, a substantially ho-mogeneous liquid of the reactants is conveyed by tubing through a minimum of two temperature zones in the presence of a sufficient amount of a surface absorbing polymer to both condition the tubing and protect the catalyzing enzyme so as to increase production, efficiency and minimize down time needed to regenerate the system for further use. In particular, a block copolymer of ethylene oxide and propylene oxide is used.

L'invention concerne un méthode de synthèse de production industrielle efficace et à extrêmement rapide de substances organiques à poids moléculaire élevé, comme de l'ADN, par des procédés requérant des environnements à plusieurs températures. Dans une opération de réaction en chaîne de polymérase, un liquide sensiblement homogène constitué des réactifs passe dans une tubulure à travers au moins deux zones de température en présence d'une quantité suffisante d'un polymère absorbant en surface afin de conditionner la tubulure et de protéger l'enzyme catalysante de manière à augmenter le temps de production, l'efficacité et le temps d'arrêt minimum nécessaires pour régénérer le système pour une utilisation supplémentaire. En particulier, un copolymère séquencé d'oxyde d'éthylène et d'oxyde de propylène est utilisé.

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