Method for accessing microbial diversity

Details Method for accessing microbial diversity Method for accessing microbial diversity

C - Chemistry, Metallurgy

12

N

C12N 1/38 (2006.01) C12N 1/04 (2006.01) C12Q 1/04 (2006.01) C12Q 1/37 (2006.01) C12Q 1/44 (2006.01)

Patent

CA 2475448

A method of interfering with quorum sensing regulation of genes to promote cell growth is disclosed. The method of is aimed at accessing microbial biodiversity. The method involves obtaining an invironmental sample comprising at least one novel (uncultivated in the laboratory) microorganism, contacting the environmental sample with an effective amount of an agent or combination of agents which interferes with the quorum sensing regulation of genes, growing the treated sample in a culture medium containing the quorum sensing signal disrupting agent or agents, and analyzing the colonies of microorganisms grown to demonstrate genetic novelty.

L'invention concerne un procédé d'interférence dans une régulation de détection de quorum de gènes pour promouvoir la croissance cellulaire. Ce procédé vise à accéder à une biodiversité microbienne. Ce procédé consiste à obtenir un échantillon environnemental comprenant au moins un nouveau micro-organisme (non cultivé en laboratoire), à mettre en contact l'échantillon environnemental avec une dose efficace d'un agent ou une combinaison d'agents interférant dans la régulation de détection de quorum de gènes, à cultiver l'échantillon traité dans un support de culture contenant l'agent ou les agents d'interruption de signal de détection de quorum, et à analyser les colonies de micro-organismes cultivées pour démontrer cette nouveauté génétique.

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