C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 11/02 (2006.01) C07K 14/315 (2006.01) C07K 14/735 (2006.01) C12N 9/12 (2006.01) C12N 9/96 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2220427
The invention provides a method of activating a reversibly inactivated enzyme or catalytic fragment or mutant thereof, wherein the enzyme is inactivated by attachment to an immobilizing moiety and is activated by release from said moiety, particularly heat-activated release and activation of enzymes, particularly for use in nucleic acid amplification techniques, the immobilized enzymes and kits for performing methods of the invention.
L'invention concerne un procédé pour activer une enzyme, un mutant ou un fragment catalytique de celle-ci, après une inactivation réversible. L'inactivation se fait par fixation à une fraction d'immobilisation et l'activation se fait par libération de cette fraction, en particulier par libération sous l'effet de la chaleur et activation des enzymes. Le procédé de l'invention est utile en particulier dans les techniques d'amplification d'acides nucléiques. L'invention concerne également des enzymes immobilisées et des nécessaires prêts à l'emploi permettant de mettre en oeuvre le procédé en question.
Lundeberg Joakim
Uhlen Mathias
Dynal A/s
Kirby Eades Gale Baker
LandOfFree
Method for activating a reversibly inactivated immobilized... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for activating a reversibly inactivated immobilized..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for activating a reversibly inactivated immobilized... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1951055