C - Chemistry – Metallurgy – 08 – G
Patent
C - Chemistry, Metallurgy
08
G
402/2, 260/212,
C08G 83/00 (2006.01) B01D 15/08 (2006.01) B01J 20/32 (2006.01) C07K 1/22 (2006.01) C07K 14/765 (2006.01) C07K 16/06 (2006.01) C08B 37/00 (2006.01) C08F 8/30 (2006.01) C08G 85/00 (2006.01) C12N 9/00 (2006.01) C12N 11/06 (2006.01)
Patent
CA 2005917
METHODS FOR ACTIVATING POLYMERIC CARRIERS AND COMPOSITIONS PREPARED THEREFROM FOR USE IN AFFINITY CHROMATOGRAPHY Abstract of the Disclosure Methods of activating polymeric carriers and of covalently binding organic ligands to such activated polymeric carriers, a novel class of products prepared thereby and the use of such products for separation and purification of organic compounds, particularly those of biochemical interest, are disclosed. In one of its aspects, the invention relates to a method of activating polymeric gels and covalently binding organic ligands containing one or more nucleophilic group such as amino, hydroxyl or sulfhydryl groups to such activated polymeric gels. In another of its aspects, the present invention relates to methods and compositions for purification and recovery of specific organic molecules of biological interest, and in particular proteins such as immunoglobulins, through the use of a non-peptido, Protein A and/or Protein G mimetic ligand (i.e., immunoglobulin-binding ligand) bound to a polymeric gel, and to methods for preparation of such nonpeptido, Protein A and/or Protein G mimetic ligands via the activated polymers.
Sim & Mcburney
Unisyn Fibertec Corporation
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