Method for adjusting the quantification range of individual...

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G01N 33/543 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2546922

The invention provides general methods for adjusting the inherent quantification range of a particular set of analytes in assays that employ capture reagents immobilized on solid supports. Specifically, the quantification range of a given analyte is adjusted to higher concentration regions by the addition of free capture reagent specific for that analyte, leaving the range of the remaining analytes the same and thereby permitting the simultaneous and accurate quantification of a plurality of analytes over a wide range of concentration values.

L'invention concerne des procédés généraux d'ajustement de la plage de quantification inhérente d'un ensemble particulier d'analytes dans des essais utilisant des réactifs de capture immobilisés sur des supports solides. La plage de quantification d'un analyte donnée est ajustée par rapport à des zones de concentrations plus élevées par l'addition de réactifs de capture libre spécifiques dudit analyte, la plage d'analytes restants étant ainsi la même et la quantification simultanée et précise d'une pluralité d'analytes sur une grande plage de valeurs de concentration étant ainsi possible.

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