A - Human Necessities – 61 – K
Patent
A - Human Necessities
61
K
A61K 48/00 (2006.01) A61K 9/66 (2006.01) A61K 31/70 (2006.01)
Patent
CA 2271582
Treatment or prevention of a disease characterized by aberrant expression of a gene in a mammalian subject is achieved using lipid-encapsulated therapeutic nucleic acid particles made from a therapeutic nucleic acid component that hybridizes specifically with the aberrantly expressed gene to reduce its expression in the subject encapsulated within a lipid particle. The lipid particle is formed from a lipid mixture including a steric- barrier lipid component selected from among lipids that prevent particle aggregation during lipid-nucleic acid particle formation and which exchange out of the lipid particle at a rate greater than PEG-CerC20, and is administered in a therapeutically effective or prophylactic amount to the mammalian subject in a plurality of separate doses separated in time by intervals of up to eight weeks. The particles are preferably formulated using a PEG-modified or polyamide oligomer-modified lipid as the first lipid component. To achieve high loading levels, an effective method for preparation of the lipid-encapsulated nucleic acid particles involves the use of a lipid mixture comprising at least the first lipid component and a protonatable lipid component selected from among lipids containing a protonatable group that has a pKa such that the lipid is in a cationic form at a pH below the pKa and a neutral form at around physiological pH. The method the invention is particularly useful for therapy using lipid-encapsulated antisense nucleic acids or ribozymes. In a more general sense, however, the method of the invention may also be used for delivery of non-cytotoxic lipid-encapsulated therapeutic agents generally in a repeat dosing regimen.
Ansell Steven M.
Cullis Pieter R.
Harasym Troy
Hope Michael J.
Klimuk Sandra K.
Ansell Steven M.
Cullis Pieter R.
Harasym Troy
Hope Michael J.
Klimuk Sandra K.
LandOfFree
Method for administration of therapeutic agents, including... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for administration of therapeutic agents, including..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for administration of therapeutic agents, including... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-2008718