Method for affinity chromatography of antithrombin iii

B - Operations – Transporting – 01 – D

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B01D 15/38 (2006.01) B01J 20/22 (2006.01) B01J 20/28 (2006.01)

Patent

CA 2616132

The invention concerns an affinity chromatography column comprising the ATIII protein bound to a solid support, characterized in that the ATIII protein has been first activated by incubation with an unmodified low-molecular weight heparin rich in active species, and in that it is covalently bound to a resin in a ratio less than about 2 mg of protein per ml of hydrated resin.

La présente invention a pour objet une colonne de chromatographie d'affinité comprenant la protéine ATIII liée à un support solide, caractérisée en ce que la protéine ATIII a été préalablement activée par incubation avec une héparine de bas poids moléculaire (HBPM) non modifiée et riche en espèces actives, et qu'elle est liée de façon covalente à une résine dans un rapport inférieur à environ 2 mg de protéine par ml de résine hydratée.

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