A - Human Necessities – 61 – K
Patent
A - Human Necessities
61
K
167/139
A61K 39/395 (2006.01) C07K 16/06 (2006.01)
Patent
CA 1193195
METHOD FOR AFFINITY PURIFICATION OF HYBRIDOMA ANTIBODIES Abstract of the Disclosure An efficient process design for purifying large quan- tities of specific antibodies by affinity chromatography is disclosed. By utilizing the process strategy described, large scale production of highly purified hybridoma antibodies and other proteins becomes feasible. The initiation of the process centers on production of hybridoma proteins which bind to a commonly available inexpensive protein such as human serum albumin (HSA) with which large quan- tities of a protein such as mouse immunoglobulin can be isolated by affinity chromatography. The mouse immunoglobulin is cova- lently linked to an inert matrix, such as Sepharose beads and this affinity reagent is used to purify a large quantity of a hydridoma protein such as rat anti-mouse IgG antibody. This rat anti-mouse IgG antibody can then be linked to sepharose beads and can be used as a general affinity reagent for the purification of any mouse immunoglobulin.
405551
Beth Israel Hospital Association (the)
Osler Hoskin & Harcourt Llp
LandOfFree
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