Method for alteration detection

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 9/12 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2441021

Methods are provided for detecting an alteration or the absence of an alteration in a target nucleic acid. Methods of the invention are useful for detecting and identifying mutations that are indicative of disease or the predisposition for disease. Methods of the invention involve annealing a series of probes to a target nucleic acid and detecting the presence of a mutation in said target nucleic acid if one of the probes fails to anneal, thereby exposing a single stranded stretch of nucleic acid that is susceptible to degradation.

L'invention concerne des méthodes permettant de détecter la présence ou l'absence d'une altération dans un acide nucléique cible. Les méthodes de l'invention sont utiles pour détecter et identifier des mutations révélant une maladie ou une diathèse. Les méthodes de l'invention consistent en outre à renaturer une série de sondes relativement à un acide nucléique cible, puis à détecter la présence d'une mutation dans ledit acide nucléique cible, si une des sondes échoue à la renaturation découvrant ainsi une extension monocaténaire d'acide nucléique susceptible de détérioration.

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