C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) G01N 33/532 (2006.01) G01N 33/58 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 2363703
Disclosed are reagents and a method for effective in vitro amplification of bio-assay signals. The method makes use of a pair of "end-to-end" complementary oligonucleotide primers to continuously form a double-stranded and highly repetitive hybrid molecule. Since this hybrid molecule is covalently linked and added to the probing molecule in the bioassays, and is also compatibly labeled, it can amplify the detection signals hundreds of times within a short period. The method can have very broad applications in bioassays using nucleic acid hybridization, immunochemical detection, and other specific interactions between two molecules or between molecules and cells.
Royce Technologies Llc A. Nevada Corporation
Thompson Lambert Llp
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