Method for amplification of nucleic acids

Details Method for amplification of nucleic acids Method for amplification of nucleic acids

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2381958

Disclosed is a probe molecule comprising single stranded nucleic acid; said probe comprising a single stranded sequence complementary to a target nucleic acid sequence; a single strand of an RNA polymerase promoter sequence; and a blocking moiety adjacent or substantially adjacent to the promoter sequence, a method of detecting a nucleic acid sequence of interest using the probe; and kits comprising the probe.

L'invention concerne une molécule sonde renfermant un acide nucléique monocaténaire. Cette sonde renferme une séquence monocaténaire complémentaire à une séquence cible d'acide nucléique, un seul brin d'une séquence promotrice d'ARN polymérase, et une fraction bloquante adjacente ou sensiblement adjacente à la séquence promotrice. L'invention concerne également un procédé de détection d'une séquence d'acide nucléique d'intérêt au moyen de la sonde, ainsi que des kits comprenant la sonde.

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