G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/543 (2006.01) G01N 33/558 (2006.01) G01N 33/563 (2006.01)
Patent
CA 2198958
Disclosed is an improvement to a sandwich type immunoassay in which there is immobilized to a solid support an antibody which is specific to an epitope of the analyte whose presence or concentration is being sought and a first labeled antibody which is specific to another epitope of the analyte. The improvement involves providing a second labeled antibody which is specific to the first labeled antibody to thereby form a chain of two or more labeled antibodies which results in amplification of the signal generated upon formation of the sandwich.
On divulgue une amélioration d'un immunodosage de type sandwich dans lequel un anticorps est immobilisé sur un support solide, anticorps qui est spécifique d'un épitope de l'analyte dont on cherche à déterminer la présence ou la concentration et un premier anticorps marqué qui est spécifique d'un autre épitope de l'analyte. L'amélioration fait intervenir un second anticorps marqué qui est spécifique du premier anticorps marqué pour ainsi former une chaîne de deux anticorps marqués ou plus qui résulte en une amplification du signal généré au moment de la formation du sandwich.
Corporation Bayer
Osler Hoskin & Harcourt Llp
LandOfFree
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