C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/00 (2006.01) C07K 14/00 (2006.01) C07K 14/245 (2006.01) C07K 14/395 (2006.01) C12N 15/67 (2006.01) C12N 15/81 (2006.01) C12N 15/90 (2006.01)
Patent
CA 2346501
A method for amplifying a foreign gene which comprises locating a hot spot region of recombination and an autonomously replicating sequence close to the foreign gene having been recombinationally transferred and providing a protein having an activity of inhibiting the progress of the replication fork formation.
L'invention concerne une méthode d'amplification d'un gène étranger consistant à placer une région de recombinaison de type point chaud et une séquence à réplication autonome à proximité du gène étranger transféré par recombinaison, et à utiliser une protéine inhibant le développement de la formation de fourches de réplication.
Horiuchi Takashi
Kobayashi Takehiko
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Horiuchi Takashi
Kyowa Hakko Kogyo Co. Ltd.
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