Method for amplifying foreign genes

C - Chemistry – Metallurgy – 12 – N

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C12N 15/00 (2006.01) C07K 14/00 (2006.01) C07K 14/245 (2006.01) C07K 14/395 (2006.01) C12N 15/67 (2006.01) C12N 15/81 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2346501

A method for amplifying a foreign gene which comprises locating a hot spot region of recombination and an autonomously replicating sequence close to the foreign gene having been recombinationally transferred and providing a protein having an activity of inhibiting the progress of the replication fork formation.

L'invention concerne une méthode d'amplification d'un gène étranger consistant à placer une région de recombinaison de type point chaud et une séquence à réplication autonome à proximité du gène étranger transféré par recombinaison, et à utiliser une protéine inhibant le développement de la formation de fourches de réplication.

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