C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/75 (2006.01) C12P 21/02 (2006.01)
Patent
CA 2719314
Certain aspects of this disclosure relate to a method of amplifying a genomic locus. In certain embodi-ments, the method may comprise: a) contacting a popula-tion of bacterial host cells with an inhibitor of an essential enzyme, where the bacterial host cells comprise a genomic locus of the structure: A1-P-M-A2, where A1 and A2 are di-rect repeats, P comprises a coding sequence for a polypep-tide, and M comprises a coding sequence for the essential enzyme; and b) selecting for cells that are resistant to the inhibitor; wherein cells that are resistant to the inhibitor have multiple copies of the amplification unit.
La présente invention concerne dans certains aspects un procédé damplification dun locus génomique. Dans certains modes de réalisation, le procédé peut comprendre : a) la mise en contact dune population de cellules hôtes bactériennes avec un inhibiteur dune enzyme essentielle, les cellules hôtes bactériennes comprenant un locus génomique ayant la structure : A1-P-M-A2, où A1 et A2 sont des répétitions directes, P comprend une séquence codant pour un polypeptide, et M comprend une séquence codant pour lenzyme essentielle; et b) la sélection des cellules qui sont résistantes à linhibiteur; les cellules qui sont résistantes à linhibiteur ayant des copies multiples du motif damplification.
Ferrari Eugenio
Peres Caroline
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Danisco Us Inc.
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