Method for analyzing amplified nucleic acids

Details Method for analyzing amplified nucleic acids Method for analyzing amplified nucleic acids

B - Operations, Transporting

01

L

B01L 3/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2655518

The invention relates to a method for analysing nucleic acids in a microfluidic device. Said method consists of the following steps: a) nucleic acids are amplified in a first chamber in the microfluidic device; b) the amplified nucleic acids are brought into contact with an additive comprising: i) monovalent cations and ii) an Mg2+ ion-binding agent, the additive being provided in a second chamber in the microfluidic device; and c) the amplified nucleic acids are hybridised on at least one probe oligonucleotide.

L'invention concerne un procédé d'analyse d'acides nucléiques dans un dispositif microfluidique, comportant les étapes suivantes: a) amplification d'acides nucléiques dans une première chambre dans le dispositif microfluidique; b) mise en contact des acides nucléiques amplifiés avec un additif contenant i) des cations monovalents et ii) un agent liant des ions Mg2+, l'additif étant contenu dans une deuxième chambre dans le dispositif microfluidique; et c) hybridation des acides nucléiques amplifiés sur au moins un oligonucléotide sonde.

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