Method for analyzing nucleic acid sequences

Details Method for analyzing nucleic acid sequences Method for analyzing nucleic acid sequences

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2397844

The invention relates to a method for analyzing nucleic acid sequences by carrying out the following steps: a) hybridizing nucleic acid fragments on complementary sequences, which are immobilized on coded supports; b) hybridizing probes on the nucleic acid fragments that are hybridized in step a); c) sequentially identifying the coded supports and analyzing the probes bound thereto in a mass spectrometer; d) assigning the obtained mass information to the sequences of the utilized probes, and; e) matching the information obtained in such a manner with a database. The inventive method permits the analysis of DNA or RNA and, in particular, the coupling of a highly parallelizable sample preparation method with an analysis method having a high output.

Procédé d'analyse de séquences d'acide nucléique, qui consiste (a) à hybrider des fragments d'acide nucléique avec des séquences complémentaires immobilisées sur des supports codés, (b) à hybrider des sondes aux fragments d'acide nucléique hybridés dans l'étape (a), (c) à identifier de manière séquentielle les supports codés et à analyser les sondes liées à ces supports dans un spectromètre de masse, (d) à attribuer les informations de masse obtenues aux séquences des sondes utilisées et (e) à aligner les informations ainsi obtenues sur une banque de données. Le procédé décrit permet l'analyse d'ADN ou d'ARN et en particulier le couplage d'un procédé de préparation de sondes hautement parallélisable avec un procédé d'analyse à haut rendement.

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