Method for analyzing translation-controlled gene expression

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C12M 1/00 (2006.01) C12M 1/34 (2006.01) C12N 15/09 (2006.01) C12Q 1/02 (2006.01) G01N 33/53 (2006.01) G01N 33/58 (2006.01) G01N 37/00 (2006.01)

Patent

CA 2468409

The invention relates to a method for analyzing gene expression, enabling a reliable correlation of the amount of mRNA transcribed by the gene to be examined, taking into account the translation state present in a cell type, tissue or organism, with the amount of protein which is translated from said mRNA. Determination of the translation efficiency of all mRNA variants which are transcribed by a gene to be examined, coding for a specific protein enables inter alia identification of the preferentially translated mRNA variant in a specific cell type, tissue or organism. It is possible to make a reliable forecast of the amount of protein expressed in a cell type, tissue or organism on the basis of the amount and translation efficiency of the preferentially translated mRNA variant coding for a protein to be examined.

La présente invention concerne un procédé d'analyse de l'expression de gènes qui permet d'effectuer une corrélation fiable de la quantité d'ARNm transcrite par un gène à analyser avec la quantité protéique résultant de la translation par cet ARNm, en prenant en considération l'état de translation dans un type de cellule, un tissu ou un organisme. La détermination de l'efficacité de translation de toutes les variantes d'ARNm transcrites par un gène à analyser qui codent pour une protéine donnée, permet entre autres l'identification de la variante d'ARNm résultant de la translation préférentielle dans un type de cellule, un tissu ou un organisme donné. La quantité et l'efficacité de translation de la variante d'ARNm résultant de la translation préférentielle, codant pour une protéine à analyser, permettent d'obtenir une prévision fiable de la quantité de protéine exprimée dans un type de cellule, un tissu ou un organisme.

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