G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/564 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2293677
The invention relates to a technology by which antibodies directed to sources of infection in body fluids can be assayed with high accuracy, expediency and specificity. More particularly, the invention provides an antibody immunoassay method in which the antigen-antibody reaction between a target antibody in a sample and an assay antigen is conducted in the presence of an E. coli component and an antibody assay method which comprises using a reagent having a specific affinity for the Fc region of an antibody IgG as the antibody assay reagent. The invention further provides an antibody assay device comprising a solid-phase support having at least (a) a first region to which a sample is applied and (b) a second region where the antibody in the sample is reacted as disposed in such an arrangement that the sample is wicked from the first region to the second region and a labeling means for detection of the result of the reaction in the second region, characterized in that the (b) second region is provided with (i) a test site in which a ligand for capturing the target antibody to be assayed is immobilized and (ii) a control site in which a ligand for capturing an arbitrary antibody occurring in the sample is immobilized.
L'invention concerne des procédés servant à détecter précisément, de façon appropriée et spécifique un anticorps dirigé contre une source d'infection dans un fluide corporel. Plus particulièrement, l'invention concerne un procédé permettant de détecter immunologiquement un anticorps par la mise en oeuvre d'une réaction antigène-anticorps entre l'anticorps cible d'un échantillon et un antigène, en présence d'un composé provenant d'Escherichia coli; et un procédé permettant de tester un anticorps au moyen d'un réactif de détection d'anticorps qui présente une spécificité de liaison pour la région Fc de l'anticorps IgG.
Katsuragi Kiyonori
Machikawa Fusaichi
Noda Atsunari
Tachikawa Tetsuya
Takahashi Shigeo
Marks & Clerk
Otsuka Pharmaceutical Co. Ltd.
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