C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/15.1, 150/3.
C12Q 1/26 (2006.01) G01N 33/66 (2006.01)
Patent
CA 1306172
ABSTRACT OF THE DISCLOSURE This invention relates to a novel method for assaying 1,5-anhydroglucitol, which is expected to serve as a marker for diabetes, and a kit therefor. More particularly, it relates to: (1) a method for assaying 1,5-anhydroglucitol, which comprises selectively removing sugars from a specimen containing 1,5- anhydroglucitol; allowing pyranose oxidase or L-sorbose oxidase to act on 1,5- anhydroglucitol contained in the sample thus obtained in the presence of oxygen; and determining 1,5-anhydroglucitol from the amount of the hydrogen peroxide thus formed; and (2) a reagent kit for assaying 1,5-anhydro- glucitol which comprises an agent for removing sugars, a reagent for detecting hydrogen peroxide and an enzyme for oxidizing 1,5-anhydroglucitol.
547332
Akanuma Hiroshi
Kato Kazuo
Masuda Minoru
Nakamura Tsuneo
Yabuuchi Masahiko
Fetherstonhaugh & Co.
Nippon Kayaku Kabushiki Kaisha
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