C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/00 (2006.01) C12Q 1/37 (2006.01) G01N 33/579 (2006.01)
Patent
CA 2136065
ABSTRACT OF THE DISCLOSURE A method for assaying an activity of a prophenoloxidase activating enzyme (PPAE), comprising assaying at least X-Arg or Y produced upon contact of the PPAE with a peptide chain of the formula X - Arg - Y wherein X is an optionally labeled amino acid residue or peptide residue, having an optionally protected .alpha.-amino group or N- terminal, provided that the amino acid residue adjoining Arg is not Gly or Ala, and Y is an organic residue capable of binding to a carboxyl group of Arg by acid amide bonding or ester bonding, or an optionally labeled amino acid residue or peptide residue, having an optionally protected .alpha.-carboxyl group or C- terminal, the peptide chain capable of being hydrolyzed into X- Arg and Y by a PPAE derived from an insect. According to the present invention, PPAE activity can be quantitatively assayed with precision and a highly precise method for determining .beta.- 1,3-glucan and/or peptidoglycan, wherein said PPAE activity is used as an index, can be provided. In addition, the present invention enables detection of fungi and bacteria without multiplication by culture, which makes early diagnosis of microbial infections possible. The present invention is applicable to a wide range of use such as tests for microbial contamination of water and food, safety tests of therapeutic agents such as antibiotics and injectable preparations, etc. 4 5
Ashida Masaaki
Hirayasu Kazunari
Kawabata Tomohisa
Tsuchiya Masakazu
Fetherstonhaugh & Co.
Wako Pure Chemical Industries Ltd.
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