Method for assaying biological sample component

Details Method for assaying biological sample component Method for assaying biological sample component

G - Physics

01

N

G01N 33/92 (2006.01) C12Q 1/44 (2006.01) C12Q 1/60 (2006.01)

Patent

CA 2363570

A method for quantitating a specific component in lipoproteins contained in a biological sample, for example, HDL (high-density lipoprotein), LDL (low- density lipoprotein) or VLDL (very low-density lipoprotein) by using a commonly employed automatic analyzer without centrifuging or making the reaction liquor cloudy due to complexes or aggregates. Namely, a controlling means, whereby an enzyme reaction can be carried out exclusively for the target component, is introduced into a method for enzymatically assaying a component in a specific lipoprotein fraction in the serum, thereby specifically assaying the component.

La présente invention concerne un procédé permettant un dosage quantitatif d'un composant spécifique dans des lipoprotéines présentes dans un échantillon biologique tel que les lipoprotéines haute densité (HDL), les lipoprotéines basse densité (LDL) ou les lipoprotéines très basse densité (VLDL). On utilise à cet effet un analyseur automatique d'un type couramment employé, sans centrifugation ni trouble de la liqueur de réaction par des complexes ou agrégats. En l'occurrence, on prend un dispositif permettant une réaction enzymatique au profit du seul composant cible, et on le fait participer à un dosage enzymatique d'un composant dans une fraction de lipoprotéine sérique spécifique, ce qui permet spécifiquement un dosage du composant.

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