Method for assaying immunosuppressant

Details Method for assaying immunosuppressant Method for assaying immunosuppressant

G - Physics

01

N

G01N 33/53 (2006.01) G01N 33/15 (2006.01) G01N 33/543 (2006.01) G01N 33/94 (2006.01)

Patent

CA 2185105

This invention relates to a method and kit for assaying an immunosuppressant having a calcineurin-inhibiting activity by assaying a complex containing immunophilin, calcineurin, calmodulin, calcium ions and a calcineulin-inhibiting immunosuppressant. According to the invention, it is possible to determine more accurately the total concentration of the substances actually having the immunosuppressant effect in the determination of the level of calcineulin-inhibiting immunosuppressants such as FK506 or cyclosporin A in blood.

La présente invention concerne un procédé et une trousse de dosage d'un immunosuppresseur présentant une activité inhibitrice de la calcineurine. Le procédé consiste à faire le dosage d'un complexe à base d'immunophiline, de calcineurine, de calmoduline, d'ions calcium et d'un immunosuppresseur inhibiteur de la calcineurine. D'après ce procédé et avec cette trousse, il est possible de déterminer plus précisément la teneur totale des substances présentant effectivement un effet immunosuppresseur en évaluant le niveau d'immunosuppresseurs inhibiteurs de la calcineurine tels que le FK506 ou la cyclosporine A.

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