Method for assaying nucleic acids by fluorescence

Details Method for assaying nucleic acids by fluorescence Method for assaying nucleic acids by fluorescence

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2660413

The invention relates to a method for determining the amount of nucleic acid present in a sample, wherein: - a fluorophore is added to the sample, - fluorescence intensities emitted by the fluorophore at at least two emission wavelengths in response to light stimulations at at least two excitation wavelengths respectively are measured, and - the amount of nucleic acid present in the sample is deduced from the measured fluorescence intensities.

L'invention concerne un procédé pour déterminer la quantité d'acide nucléique présente dans un échantillon, dans lequel : un fluorophore est ajouté à l'échantillon ; les intensités de fluorescence émises par le fluorophore à au moins deux longueurs d'onde d'émission en réponse à des stimulations de lumière à au moins deux longueurs d'onde d'excitation respectivement sont mesurées ; et la quantité d'acide nucléique présente dans l'échantillon est déduite des intensités de fluorescence mesurées.

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