Method for assaying protein-protein interaction

Details Method for assaying protein-protein interaction Method for assaying protein-protein interaction

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 14/47 (2006.01) C07K 14/705 (2006.01) C07K 19/00 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/50 (2006.01) C12N 15/12 (2006.01) C12N 15/57 (2006.01) C12N 15/63 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2531698

The invention relates to a method for determining if a test compound, or a mix of compounds, modulates the interaction between two proteins of interest. The determination is made possible via the use of two recombinant molecules, one of which contains the first protein a cleavage site for a proteolytic molecules, and an activator of a gene. The second recombinant molecule includes the second protein and the proteolytic molecule. If the test compound binds to the first protein, a reaction is initiated whereby the activator is cleaved, and activates a reporter gene.

L'invention concerne une méthode permettant de déterminer si un composé ou un mélange de composé testés modulent l'interaction entre deux protéines étudiées. Cette détection est mise en oeuvre par l'intermédiaire de deux molécules recombinées, dont l'une contient la première protéine, un site de clivage pour une molécule protéolytique, et un activateur d'un gène. La seconde molécule recombinée contient la seconde protéine et la molécule protéolytique. Si le composé testé se lie à la première protéine, une réaction entraînant le clivage l'activateur et l'activation d'un gène rapporteur par ce dernier, est déclenchée. .

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