Method for assembly of a polynucleotide encoding a target...

C - Chemistry – Metallurgy – 07 – H

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Details

C07H 21/00 (2006.01) C12N 15/10 (2006.01) C12N 15/66 (2006.01) C12P 19/34 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2454561

The present invention provides a novel approach to utilizing the results of genomic sequence information by computer-directed polynucleotide assembly based upon information available in databases such as the human genome database. Specifically, the present invention can be used to select, synthesize and assemble a novel, synthetic target polynucleotide sequence encoding a target polypeptide. The target polynucleotide can encode a target polypeptide that exhibits enhanced or altered biological activity as compared to a model polypeptide encoded by a natural (wild-type) or model polynucleotide sequence.

La présente invention concerne une nouvelle approche d'utilisation des résultats d'informations de séquences génomiques par le biais d'un assemblage de polynucléotides informatisé reposant sur des informations disponibles dans des bases de données, telles que la base de données du génome humain. Plus spécifiquement, la méthode de cette invention peut être utilisée pour sélectionner, synthétiser et assembler une nouvelle séquence de polynucléotides cible synthétique codant un polypeptide cible. Ce dernier peut coder un polypeptide cible qui présente une activité biologique améliorée ou modifiée en comparaison avec un polypeptide type codé par une séquence de polynucléotides type ou naturelle (de type sauvage).

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