Method for assessing cancerous state of mammal-derived specimen

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) A61K 45/00 (2006.01) A61P 35/00 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2458182

A method of evaluating the degree of canceration of a mammal-origin specimen characterized by having: (1) the first step of measuring the methylation frequency or an indication correlating thereto of Heparan sulfate D- glucosaminyl-3-O-sulfotransferase gene contained in the mammal-origin specimen; and (2) the second step of comparing the methylation frequency or an indication correlating thereto measured above with a control and judging the degree of canceration of the specimen based on the thus obtained difference.

La présente invention concerne un procédé permettant l'évaluation du niveau de cancérisation d'un prélèvement mammalien. Ce procédé se caractérise par ses deux opérations. La première opération (1) consiste à mesurer la fréquence de méthylation, ou une indication en corrélation avec elle, concernant le gène de la D-glucosaminyle-3-O-sulfotransférase du sulfate d'héparane dans le prélèvement mammalien. La seconde opération (2) consiste à comparer la fréquence de méthylation, ou une indication en corrélation avec elle, mesurée en excès d'un témoin, puis à déterminer le niveau de cancérisation du prélèvement sur la base de la différence ainsi obtenue.

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