Method for assessing the fertility of male mammals and kit...

Details Method for assessing the fertility of male mammals and kit... Method for assessing the fertility of male mammals and kit...

C - Chemistry, Metallurgy

12

Q

150/13

C12Q 1/02 (2006.01) G01N 33/50 (2006.01)

Patent

CA 1249501

METHOD FOR ASSESSING THE FERTILITY OF MALE MAMMALS AND KIT THEREFOR Abstract of the Disclosure A method for evaluating the fertility of a male mammal. A semen sample is obtained from the individual to be tested. The sperm is separated from the seminal plasma of the semen sample. At least one test portion and a control portion of the sperm are isolated. The sperm portions are incubated, the test portion being exposed to a glycosaminoglycan in a concentration effective to induce an acrosome reaction in sperm. A representative sample of incubated sperm from each portion is then counted by means of observation by light microscopy to measure the increase in acrosome reaction in the test portions as compared to the control portion. A kit is provided for the convenient performance of the method. The kit includes a supply of sterile liquid culture medium, sterile closable culture vessels, and a supply of selected, sterile GAG meterable substantially aseptically in known amounts into each culture vessel used as a test vessel.

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