Method for biotransformation of the clyclosporin compound...

C - Chemistry – Metallurgy – 12 – P

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C12P 21/04 (2006.01) C12Q 1/04 (2006.01) C12Q 1/25 (2006.01)

Patent

CA 2591781

A method of producing metabolites of a xenobiotic compound by biotransformation using a microorganism, wherein the xenobiotic compound is cyclosprorm ISA247, which is delivered to the microorganism in a mixture with a surfactant The method can be scaled up to produce large quantities of metabolites by, for example, biotransformation in a reactor The metabolites produced by the present method can be used for antibody production, as standards in therapeutic dose monitoring, or in pharmaceutical applications

L'invention concerne un procédé permettant de produire des métabolites d'un composé xénobiotique par biotransformation au moyen d'un micro-organisme, le composé xénobiotique étant la cyclosporine ISA247, qui est administré au micro-organisme en mélange avec un tensioactif. Ce procédé peut être étendu à grande échelle de manière à permettre la production de grandes quantités de métabolites, par exemple par biotransformation dans un réacteur. Les métabolites produits par ce procédé peuvent servir à la production d'anticorps, servir de métabolites de référence pour le contrôle des doses thérapeutiques, ou être utilisés des applications pharmaceutiques.

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