Method for calibrating machines for the analysis of...

G - Physics – 01 – N

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G01N 15/05 (2006.01) G01N 21/82 (2006.01)

Patent

CA 2620676

A method for calibrating machines (10) suitable to effect an analysis of a blood sample (12) by means of measuring the erythrocyte sedimentation rate (ESR) and/or aggregation of the red corpuscles, wherein said measurement is effected by exploiting the optical density kinetic obtained from the measurement of the variation in the optical density of the blood sample (12) in an interval of time, comprises a measuring step in which, by means of the same machine (10) with which the measurement of the optical density is effected on said blood sample (12), a measurement is effected of the optical density of two latexes (23a, 23b, 23c), or torbidometric samples. Each of the two latexes (23a, 23b, 23c) has a known optical density, reproducible, measurable and different from each other. The method also comprises a comparison step, in which the difference is calculated between the values of optical density of the latexes (23a, 23b, 23c) as obtained from the measurement performed by the machine (10) and the known values of optical density, to allow to determine at least one correction factor usable to calibrate said machine (10).

Procédé de calibrage de machines (10) adapté pour l~analyse d~un échantillon de sang (12) en mesurant le taux de sédimentation des érythrocytes (ESR) et/ou l'agrégation des globules rouges, ladite mesure étant effectuée en utilisant la cinétique de densité optique obtenue depuis la mesure de la variation de densité optique de l~échantillon de sang (12) dans un intervalle de temps, comprenant une étape de mesure dans laquelle, au moyen de la même machine (10) avec laquelle la mesure de la densité optique est effectuée sur ledit échantillon de sang (12), une mesure de la densité optique de deux latex (23a, 23b, 23c), ou d'échantillons turbidimétriques, est effectuée. Chacun des deux latex (23a, 23b, 23c) présente une densité optique connue, reproductible et mesurable, et différente de celle de l~autre latex. Le procédé selon l~invention comprend également une étape de comparaison, dans laquelle la différence entre les valeurs de la densité optique des latex (23a, 23b, 23c) telles qu~elles sont obtenues dans les mesures effectuées par la machine (10) et leurs valeurs de densité optique connues est calculée afin de permettre la détermination d~au moins un facteur de correction utilisable pour calibrer ladite machine (10).

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