Method for carrying out the controlled post-harvest...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/00 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2360496

The invention relates to a method for extracting a desired protein from a transgenic host organism, whereby the expression of the gene that codes for this protein is firstly carried out after the host organism has been harvested. The inventive method is characterized in that: (a) the transgenic host organism contains the gene that codes for the desired protein in such a way that the expression thereof is firstly carried out in the presence of a chemical inductor, and; (b) the gene is brought into contact with the inductor via the phase surrounding the host organism after the host organism has been harvested. The invention also relates to a host organism that is suited for carrying out said method.

L'invention concerne un procédé d'obtention d'une protéine désirée à partir d'un organisme hôte transgénique, dans lequel l'expression du gène codant pour cette protéine ne s'effectue qu'après récolte de l'organisme hôte, caractérisé en ce que (a) l'organisme hôte transgénique renferme le gène codant pour la protéine désirée, de telle façon que son expression ne s'effectue qu'en présence d'un inducteur chimique, et en ce que (b) la mise en contact avec l'inducteur s'effectue après la récolte de l'organisme hôte, via la phase entourant l'organisme hôte. L'invention concerne en outre un organisme hôte approprié pour la mise en oeuvre de ce procédé.

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