Method for cellulase production

C - Chemistry – Metallurgy – 12 – N

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C12N 9/42 (2006.01) C12P 19/00 (2006.01) C12P 19/02 (2006.01) C12P 19/14 (2006.01)

Patent

CA 2697090

A fermentation process using hemicellulose-derived carbohydrates for the production of cellulase mixtures with a high proportion of cellulases relative to hemicellulases is provided. The cellulases produced by the process of the invention are further characterized by high specifi productivity. The resulting cellulase mixtures comprise at least two times more cellulase than hemicellulase and are useful for the hydrolysis of cellulosic substrated, particularly, pretreated lignocellulosic substrate.

L'invention concerne un procédé de fermentation dans lequel sont utilisés des carbohydrates dérivés de l'hémicellulose pour la production de mélanges de cellulase avec une proportion élevée de cellulases par rapport aux hémicellulases. Les cellulases obtenues selon le procédé de l'invention se caractérisent également par une productivité spécifique élevée. Les mélanges de cellulase obtenus comprennent au moins deux fois plus de cellulase que d'hémicellulase et sont utilisés dans l'hydrolyse de substrats cellulosiques, en particulier un substrat lignocellulosique prétraité.

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