Method for characterising analytes

Details Method for characterising analytes Method for characterising analytes

C - Chemistry, Metallurgy

07

K

C07K 7/08 (2006.01) C07K 1/13 (2006.01) G01N 27/62 (2006.01) G01N 33/58 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2480836

Provided is a method for characterising an analyte by matrix assisted laser desorption ionisation (MALDI) mass spectrometry, which method comprises: (a) labelling the analyte with a light-absorbing label that absorbs light at a pre- determined frequency, to form a labelled analyte; (b) embedding the labelled analyte in a matrix formed from at least one compound that absorbs light, to form an embedded labelled analyte; (c) desorbing the embedded labelled analyte by exposing it to light having the pre-determined frequency, to form a desorbed analyte; and (d) detecting the desorbed analyte by mass spectrometry to characterise the analyte.

L'invention concerne un procédé permettant de caractériser un analyte par spectrométrie de masse à désorption-ionisation laser assistée par matrice (MALDI). Ce procédé consiste à (a) marquer l'analyte avec un marqueur d'absorption de la lumière qui absorbe la lumière à une fréquence prédéterminée pour former un analyte marqué ; (b) encastrer l'analyte marqué dans une matrice formée à partir d'un composé au moins qui absorbe la lumière pour former un analyte marqué encastré ; (c) désorber l'analyte marqué encastré en l'exposant à la lumière présentant la fréquence prédéterminée pour former un analyte désorbé et (d) détecter l'analyte désorbé par spectrométrie de masse pour caractériser l'analyte.

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