Method for characterization of abnormal cells

Details Method for characterization of abnormal cells Method for characterization of abnormal cells

C - Chemistry, Metallurgy

12

Q

C12Q 1/04 (2006.01) G01N 33/543 (2006.01) G01N 33/574 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2275335

It is described a method to detect and phenotype target cells in cell suspensions by using particles coated with antibodies/ligands directed to antigenic determinants/receptors expressed on the target cells, characterized in that several types of particles, each particle instrumentally or visually separable by fluorescence, color and size, and each particle coated with a different antibody or ligand, are incubated simultaneously or subsequently with cell suspensions containing the target cells, in connection or not with a per se known enrichment procedure, a use of the method and a kit.

L'invention concerne une méthode de détection et de détermination du phénotype de cellules cibles dans des suspensions cellulaires au moyen de particules enrobées d'anticorps/ligants dirigés contre des déterminants antigéniques/récepteurs exprimés sur les cellules cibles, caractérisée en ce que plusieurs types de particules, chaque particule étant séparable visuellement ou au moyen d'instruments par fluorescence, couleur et grosseur, et chaque particule enrobée d'un anticorps ou d'un ligant différent, sont soumises à incubation simultanément ou successivement avec des suspensions cellulaires contenant les cellules cibles, en liaison ou non avec un mode d'enrichissement connu en soi; l'invention concerne également une utilisation de la méthode et un kit.

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