Method for classifying and counting leukocytes

Details Method for classifying and counting leukocytes Method for classifying and counting leukocytes

G - Physics

01

N

G01N 33/52 (2006.01) C12Q 1/06 (2006.01) G01N 15/14 (2006.01) G01N 33/49 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2151667

A method for classifying and counting leukocytes which includes the steps of (i) adding a first reagent used for classifying leukocytes into four groups which comprises, (a) at least one ionic surfactant in a sufficient amount to lyse erythrocytes and to damage a part of cell membrane of leuko- cytes, (b) at least one organic compound having an anionic group in a sufficient amount to bond with a cationic compo- nent present in leukocytes to give morphological differences between leukocytes, (c) a nonionic surfactant, and (d) a buffer for adjusting pH, to a part of a blood sample to determine information on the cell size and morphological features in order to classify leukocytes into four groups consisting of three groups corresponding to lymphocytes, mononuclear cells and eosinophils and one group correspond- ing to neutrophils and basophils; (ii) adding a second reagent used for measuring basophils to another part of the blood sample to determine information on at least the cell size in order to classify basophils, and (iii) classifying leukocytes based on the information obtained in the steps (i) and (ii) and counting.

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