Method for classifying leukocytes and a reagent used therefor

Details Method for classifying leukocytes and a reagent used therefor Method for classifying leukocytes and a reagent used therefor

G - Physics

01

N

150/13, 150/3

G01N 33/50 (2006.01) G01N 15/14 (2006.01)

Patent

CA 2027452

A reagent for enabling leukocytes to be classified and measured by lyzing erythrocytes and acting on leuko- cytes, which has a pH of 1.5 - 5.0, and an osmolarity of - 120 mOsm/kg and contains a polyoxyethylene based nonionic surfactant represented by the general formula: R1-R2-(CH2CH2O)n-H (where R1 is an alkyl, alkenyl or alkynyl group having 12 - 22 carbon atoms; R2 is -O-, (see formula I) or -COO-; and n is an integer of 20 - 100) or a reagent for the same purpose, which comprises a first solution which is the reagent described above and a second solution to be added to the first solution, and is a pH of 5.0 - 12.0 and has an osmolarity of 150 - 2000 mOsm/kg after addition of the second solution to the first solution; and methods for classifying leukocytes into at least three types; that is, lymphocytes, monocytes and granulocytes and fox detecting abnormal cells by using a reagent described and particulate analysis methods consisting of RF method and DC method are disclosed.

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