Method for classifying plant embryos using raman spectroscopy

A - Human Necessities – 01 – H

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A01H 1/04 (2006.01) A01C 1/02 (2006.01) G01J 3/44 (2006.01) G01N 21/31 (2006.01) G01N 21/65 (2006.01) G01N 33/483 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2470889

A three-step method for classifying plant embryo quality using Raman spectroscopy is provided. First, a classification model is developed based on Raman spectral data of reference samples of plant embryos or any portions of plant embryos of known embryo quality. The embryo quality may be known based on a comparison to a normal zygotic embryo or on actual planting of the embryo to observe its germination and subsequent growth. Then, a data analysis is carried out by applying one or more classification algorithms to the acquired Raman spectral data to develop a classification model. Second, Raman spectral data of a plant embryo or any portion of a plant embryo of unknown embryo quality are obtained. Third, the classification model developed in the first step is applied to the Raman spectral data obtained from the embryo (or any portions thereof) of unknown quality to classify the quality of this plant embryo. -11-

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