Method for cleavage of fusion proteins

Details Method for cleavage of fusion proteins Method for cleavage of fusion proteins

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) A23L 1/305 (2006.01) A61K 38/27 (2006.01) A61K 38/58 (2006.01) C07K 14/61 (2006.01) C07K 14/815 (2006.01) C12N 9/50 (2006.01) C12N 15/15 (2006.01) C12N 15/57 (2006.01)

Patent

CA 2286861

An improved method for recovering recombinantly produced polypeptides is described. The method involves expressing the recombinant polypeptide as a fusion protein with a pro-peptide. The pro-peptide-polypeptide fusion protein can be cleaved and the recombinant polypeptide released under the appropriate conditions.

Cette invention concerne un procédé amélioré permettant de récupérer des polypeptides produits de manière recombinante. Ce procédé consiste à exprimer le polypeptide recombinant sous forme d'une protéine hybride à l'aide d'un pro-peptide. La protéine hybride pro-peptide-polypeptide peut ensuite être soumise à un clivage, et le polypeptide recombinant peut être libéré dans des conditions adéquates.

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