Method for cleaving and deglycosylating antibodies to...

G - Physics – 01 – N

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G01N 33/53 (2006.01) A61K 47/48 (2006.01) G01N 33/574 (2006.01) G01N 33/76 (2006.01)

Patent

CA 2481420

The present invention details methods to detect glycoproteins in samples to identify a disease state. In particular, an improved sandwich assay is described, using both an antibody and an array of lectins. Methods for increasing the efficiency of interaction of an antibody with a ligand by cleaving the antibody to remove the carbohydrate moiety, and orienting the cleaved fragment on a matrix to enhance recognition of a ligand are also described. Methods of differentiating pregnancy-derived and disease derived samples are also described.

L'invention concerne des procédés pour détecter des glycoprotéines dans des échantillons pour identifier un état pathologique. En particulier, un essai de sandwich amélioré est décrit, lequel essai faisant appel à la fois à un anticorps et à un réseau de lectines. L'invention concerne des procédés permettant d'accroître l'efficacité d'interaction d'un anticorps et d'un ligand par le biais du clivage de l'anticorps pour retirer la fraction d'hydrate de carbone, et pour orienter le fragment clivé sur une matrice pour améliorer la reconnaissance d'un ligand. L'invention concerne encore des procédés de différenciation d'échantillon dérivés de sujets présentant une grossesse ou une maladie.

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