Method for cleaving chimeric protein using processing enzyme

Details Method for cleaving chimeric protein using processing enzyme Method for cleaving chimeric protein using processing enzyme

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 14/585 (2006.01) C07K 14/635 (2006.01) C07K 19/00 (2006.01) C12N 9/38 (2006.01)

Patent

CA 2198966

A chimeric protein is provided which comprises a target protein and has been designed so as to be easily cleaved by a processing enzyme, permitting a target protein to be efficiently recovered. The chimeric protein is represented by the following formula: A-L-B wherein A represents a protective peptide; B represents a target peptide; and L represents a linker peptide having the sequence X1-X2-(Pro, Lys, or Arg)-Arg, wherein X1 and X2 represent any amino acid, in its C-terminal region and a domain rich in His in its N-terminal region.

L'invention fournit une protéine chimère qui comprend une protéine cible et qui a été conçue pour être clivée facilement par une enzyme de traitement, permettant ainsi la récupération efficace de la protéine cible. La protéine chimère est représentée par la formule suivante : A-L-B où A représente un peptide de protection, B un peptide cible et L un peptide de liaison ayant la séquence X1-X2-(Pro, Lys ou Arg)-Arg, où X1 et X2 représentent n'importe quel acide aminé, dans sa région C-terminale et un domaine riche en His dans sa région N-terminale.

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