Method for cloning and expression of nrui restriction...

Details Method for cloning and expression of nrui restriction... Method for cloning and expression of nrui restriction...

C - Chemistry, Metallurgy

12

N

C12N 15/00 (2006.01) C07H 21/00 (2006.01) C07K 14/00 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/16 (2006.01) C12P 21/00 (2006.01) C12Q 1/44 (2006.01)

Patent

CA 2646253

Recombinant DNA encoding NruI- and Sbol-like restriction endonucleases and methylases and their amino acid sequences are provided as well as methods for expressing the enzymes in transformed host cells and purifying the enzymes.

L'invention concerne un ADN recombinant codant des endonucléases de restriction similaires à Nrul et à Sbol et des méthylases et leurs séquences d'acides aminés ainsi que des procédés pour exprimer les enzymes dans des cellules hôtes transformées et purifier les enzymes.

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