Method for continuously isolating active proteins

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 1/30 (2006.01) A23L 1/221 (2006.01) C07K 14/415 (2006.01) C12N 9/00 (2006.01)

Patent

CA 2350215

The invention concerns a method for isolating active plant material proteins or proteins from a fermenting medium which consists in continuously precipitating and in one single step in an appropriate organic solvent the active proteins contained in an enzymatic solution extracted from said plant material or from the fermenting medium, in a specific reactor, the conditions in the reactor being adjusted so as to obtain a precipitate of non-denatured proteins, said precipitate is then subjected to a maturing step before being continuously separated.

Procédé d'isolation de protéines actives de materiel végétal ou de milieu de fermentation dans lequel on fait précipiter en continu et en une seule étape dans un solvant organique approprié les protéines actives contenues dans une solution enzymatique extraite dudit matériel végétal ou du milieu de fermentation, dans un réacteur spécifique, les conditions dans le réacteur étant réglées de manière à obtenir un précipité de protéines non dénaturées, ledit précipité est ensuite passé par une étape de maturation avant d'être séparé en continu.

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