Method for controlling insects of the order diptera using a...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/32 (2006.01) A01N 63/00 (2006.01) C07K 14/325 (2006.01) C12N 1/21 (2006.01)

Patent

CA 2584753

The invention provides a method for controlling insects of the Order Diptera by providing a Bacillus thuringiensis strain or variant thereof, or a spore or crystal of the Bacillus thuringiensis strain or variant thereof, and either contacting the insect with or administering to an animal the Bacillus thuringiensis strain or variant thereof, or the spore or crystal of the Bacillus thuringiensis strain or variant thereof; or applying the Bacillus thuringiensis strain or variant thereof, or the spore or crystal of Bacillus thuringiensis strain or variant thereof to an infested area. The Bacillus thuringiensis strain contains a plasmid carrying endotoxin genes for encoding delta~ endotoxins Cry1 A, Cryl B, Cryl F, Cryl H, Cryl I, Cryl K, Cry2 or a variant thereof. Preferably, the strain is Bacillus thuringiensis strain LRC3 deposited as ATCC PTA-~6248. Methods for preparing the strain, spores, crystals, mutants, variants, and compositions incorporating same are described.

L'invention concerne un procédé permettant de combattre les insectes de l'ordre des diptères par fourniture d'une souche du Bacillus thuringiensis ou d'un variant de celui-ci, d'une spore ou d'un cristal de la souche du Bacillus thuringiensis ou d'un variant de celui-ci, par contact de l'insecte avec la souche de Bacillus thuringiensis ou un variant de celui-ci, avec sa spore ou son cristal, par administration de la souche du Bacillus thuringiensis ou d'un variant de celui-ci à un animal, ou de la spore ou du cristal de la souche du Bacillus thuringiensis ou d'un variant de celui-ci, ou par application de la souche du Bacillus thuringiensis ou d'un variant de celui-ci ou de la spore ou du cristal de la souche du Bacillus thuringiensis ou d'un variant de celui-ci à une zone infestée. La souche du Bacillus thuringiensis contient un plasmide portant des gènes d'endotoxine codant des delta-endotoxines Cry1A, CrylB, CrylF, CrylH, CrylI, CrylK, Cry2 ou un variant associé. De préférence, il s'agit de la souche du Bacillus thuringiensis LRC3 déposée sous ATCC PTA--6248. L'invention concerne des procédés de préparation de la souche, des spores, des cristaux, des mutants, des variants, ainsi que des compositions les renfermant.

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