Method for controlling the growth of cell culture

C - Chemistry – Metallurgy – 12 – N

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12N 1/04 (2006.01) C12N 1/22 (2006.01)

Patent

CA 2671076

The present invention provides a method for controlling the growth of microbes grown in a medium to high cell-densities by the fed-batch technology, wherein a two-phase system, having a liquid phase (cultivation medium) and a solid or gel phase, the solid or gel phase provides a source of substrate-delivering polymer which is turned by an enzyme in a controlled way into a growth-limiting substrate or pH adjusting agent. The present invention also provides a method for restricting the synthesis of growth-limiting metabolites and preventing oxygen-depletion in microbial cell cultures.

La présente invention concerne un procédé servant à contrôler la croissance de microbes qu'on fait croître dans un milieu jusqu'à des densités cellulaires élevées par la technologie d'alimentation discontinue (fed-batch), caractérisé par un système à deux phases, ayant une phase liquide (milieu de culture) et une phase solide ou de gel, la phase solide ou de gel fournissant une source de polymère fournissant un substrat qui est transformé par une enzyme d'une manière contrôlée en un substrat limitant la croissance ou un agent ajustant le pH. La présente invention concerne également un procédé servant à limiter la synthèse de métabolites limitant la croissance et à empêcher l'appauvrissement en oxygène dans des cultures cellulaires microbiennes.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Method for controlling the growth of cell culture does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for controlling the growth of cell culture, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for controlling the growth of cell culture will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1543024

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.